All that is known with certainty is that Francisco de Padilla was present at the 1540 muster as an unassigned horseman.
1530-1540:
?AHP, Legajo 48, Oficio 1, 1535 [fol.188v:debt: Francisco de Padilla, azemilero de azeyten; vecino of Triana; to jurado Luis de Prado; 3 Feb. (doesn't know how to write)]
1540-1550:
1540, Feb: Muster in Flint & Flint, Documents, Document 12 [1 horse, native arms]
1550-1560:
?AGI, Contaduría, 663B [Payment to conquistadores, their wives and children: to a Francisco de Padilla, son of a first conqueror, 120 pesos, in December 1554, also paid 80 pesos for the 8 months ending in August 1555, again paid 160 pesos in December 1556];
?AGI, Contaduría, 664, 1557 [Money to first conquerors yearly payments: Francisco de Padilla, son of a conqueror, 80 pesos];
?AGI, Contaduría, 664, 1558 [Payments to conquistadores, wifes and children: Francisco de Padilla, son of one of the first conquerors, 120 pesos];
?AGI, Contaduría, 664, 1559 [Payments to conquistadores, wifes and children: Francisco de Padilla, son of one of the first conquerors, 80 pesos, 6 Apr 1558-6 Apr 1559];
?AGI, Contaduría, 664, 1560 [Payments to conquistadores, wifes and children: Francisco de Padilla, son of one of the first conquerors, 80 pesos, 6 Apr 1559-6 Dec 1559];
?AGI, Patronato, 20, N.5, R.15 [payments to conquistadores and their heirs] [a Francisco de Padilla is listed as a son of an unnamed conquistador]
1560-1570:
?AGN, Instituciones Coloniales, GD72, Real Audiencia, Mercedes, vol.5-6, segunda parte, exp. s/n, 1563 [fols. 324v – 325v: January 1563: [a Francisco de Padilla is corregidor del pueblo de Xicotepeque; he is directed to go to the pueblo de Açoaque to take the residencia of the governor and other Indian officials and fols. 367r y v: January 1563: a complaint is made against Francisco de Padilla, corregidor y justicia en el pueblo de Xicotepeque, that he has sent Indian assistants with the vara de justicia to perform the functions of his office; he is instructed to do this only when necessary]
?Catálogo, Resumen e Índices [1565, Ana López, hija y heredera de Alexo Hernández y mujer legítima de Juan Pérez Calafate, albañil, vecino, otorga poder general a Tomás de Moya, solicitador, y a Hernando del Campo, procurador de causas, vecinos. No firmaron. Firmaron Juan Pérez y Juan de Escalante. Testigos: Francisco de Padilla, Juan de Escalante y Juan Ruíz, vecinos y estantes, que juraron conocer a los otorgantes y Juan Pérez]
?AGNEP, Notaría 4, Caja 4, 1570, Carpeta 1.18 [9 July 1569, Acapetlavaca, Juan de Santiago, el viejo, residente in the pueblo de Acapetlavaca agrees to pay Francisco de Padilla 24 pesos de oro común for una obra buena; and 9 July 1569, Francisco de Padilla, witness to poder in Acapetlavaca]