Here is a list of wives of male members of the expedition, about whom information is included in the data base:
- Doña Isabel de Aguilar, widow of Diego de Torres, married Rodrigo de Paz Maldonado.
- Juan de Peñas was husband to Juana de Sola de Vida or Sondevilla.
- Doña Clara de Bocanegra was married to Alonso Pérez.
- Antón Pérez Buscavida was married, but his wife's name is not known.
- Melchior Pérez de la Torre was married to three women: first to Francisca Xérez, an india, then to Ana Martel, and finally to María Álvarez.
- Juan de Plasencia had a wife whose name is unknown.
- Diego de Puelles' wife Juana de Frías remained in Spain.
- The wife of Luis Ramírez de Vargas was doña Ana de Luján.
- Rufina de Grijalva married Melchor de Robles in 1543.
- Juana de Solórzano was the wife of García Rodríguez.
- Alonso Rodríguez Parra had a wife who remained in Spain; her name is unknown.
- Inés López was married to Francisco Roxo.
- Juan Ruiz (1) married a daughter of Bartolomé Sánchez.
- An unnamed daughter of Francisco Maldonado was the wife of Marcos Ruiz de Rojas.
- Hernandarias de Saavedra's wife came from Spain with him but died shortly after; her name is not known.
- Catalina Díez de Santillana was married to Gaspar de Salamanca.
- Andrés de Salinas' wife, name unknown, lived in Burgos.
- Miguel Sánchez (2) was married to a woman whose name is not known.
- Rodrigo Sánchez's wife's name is unknown.
- María de Espinosa was married to Francisco de Santillana.
- The name of Rodrigo Simón's wife is not known.
- Alonso de Toro was married, but his wife's name is unknown.
- Francisco de Torres was married twice, but the name of neither wife is known.
- Juan Troyano was married to a Native woman of Tierra Nueva.
- Hernando de Valle's wife's name is not known.
- A Juana Morales was the wife of Francisco de Villafranca.
- Doña Leonor Saavedra was married to Juan de Villarreal.
- Martín de Villaroya's wife was Catalina de Hozes, making him a son-in-law of Alonso Sánchez and Francisca de Hozes.
- Doña María de Mendoza was the wife of Juan de Zaldívar.